Thwart cancer cells by triggering 'virus alert'
【LWBS 2015 09 02 A】(SpringRain Edited from Johns Hopkins Medicine)
Working with human cancer cell lines and mice, researchers at the Johns Hopkins Kimmel Cancer Center and elsewhere have found a way to trigger a type of immune system "virus alert" that may one day boost cancer patients' response to immunotherapy drugs. An increasingly promising focus of cancer research, the drugs are designed to disarm cancer cells' ability to avoid detection and destruction by the immune system.
In a report on the work published in the Aug. 27 issue of Cell, the Johns Hopkins-led research team says it has found a core group of genes related to both a viral defense warning system and susceptibility to a demethylating drug called 5-azacytidine that chemically alters their ability to operate through a process called demethylation.
A study with similar findings authored by Daniel De Carvalho, Ph.D., at the Ontario Cancer Institute/Princess Margaret Hospital, and Peter Jones, Ph.D., D.Sc., director of research at the Van Andel Institute, focused on the ability of DNA demethylating agents to target colorectal cancer stem cells, is published in the same journal issue.
Tumors are known to co-opt cellular gene-silencing systems that add tiny chemicals called methyl groups to areas of genes, thereby turning off the affected gene function. Such "epigenetic" control normally occurs in many genes, including ones that contain DNA leftover from previous exposures to viruses. When epigenetic control of these genes is removed, the virus-laden gene sequences are activated and trigger an alert to immune system cells that a virus has invaded.
"A main barrier to immune therapy success has been the tumor's ability to keep the immune system from functioning against the cancer," says study leader Stephen Baylin, M.D., the Virginia and Daniel K. Ludwig Professor of Cancer Research at the Kimmel Cancer Center. "The immune cells are there, but like an unarmed army, they hang around and do nothing. However, certain epigenetic processes that silence such viral defense genes can be reversed in tumor cells with a demethylating drug, making immunotherapies work more effectively to kill cancer cells."
For their new study, Baylin and his team worked with laboratory-grown cell lines from human ovarian, colon and skin cancer, and the team led by De Carvalho worked with colon cancer cells. In the cancer cell lines, both teams found that the viral defense pathway can be turned on when the cells were exposed to 5-azacytidine. Once the pathway is activated, Baylin adds, the tumor cells release signaling proteins called interferons that rouse other cancer-fighting cells in the immune system.
Then, the Johns Hopkins team created a gene signature of the viral defense pathway. In tumor samples available from the National Cancer Institute's Cancer Genome Atlas project, the scientists used the gene signature to distinguish between tumor samples with high expression of the pathway from those with low expression. Those with high expression may respond to certain immunotherapy drugs without the aid of 5-azacytidine, but those with low expression levels may need the epigenetic drug to boost response to immunotherapy, says Baylin.
Looking for the connection between the pathway's expression and immunotherapy drug response, the Johns Hopkins investigators and their colleagues focused on expression levels of the viral defense pathway in tumor cells from 21 patients with melanoma treated with the immune therapy drug ipilimumab at Memorial Sloan Kettering Cancer Center. They found high expression levels in the cells of seven of eight of those patients who had responded well to ipilimumab. Cells from all 12 patients with limited response to ipilimumab had low expression of the viral defense pathway.
In a melanoma mouse model in which ipilimumab alone was partially effective, adding 5-azacytidine to ipilimumab triggered a better tumor response.
"Our findings further decipher the mechanisms that lead to this tumor cell immune reaction and offer a way to potentially boost the success of immune therapies in patients with cancer," says Baylin, who first became interested in the immune system's connection to 5-azacytidine when laboratory research and clinical trials at Johns Hopkins hinted at the drug's ability to prevent cancer cells' proliferation when combined with immunotherapy.
Baylin and his colleagues say that, if their findings are confirmed and extended in clinical trials, the 5-azacytidine treatment could be followed by ipilimumab or other types of immunotherapy called checkpoint blockade, which lower cancer cells' defenses and allow immune system cells to see and destroy them.
"Treatment with 5-azacytidine activates interferon signaling in tumor cells and, when followed by checkpoint blockade immune therapy, the immune cells could go into increased action against the cancer," says Johns Hopkins research fellow and lead author Katherine Chiappinelli, Ph.D.
Baylin and Chiappinelli caution that clinical trials will take time to learn how effective the strategy of alerting the viral defense pathway might be. But the strategy holds promise, he says, for patients who have cancers with low expression of the pathway.
DNA Methylation Inhibitors in Cancer Therapy: The Immunity Dimension
An Immune Facet of the Cellular Response to DNA Demethylating Agents
DNA demethylating agents allow bidirectional transcription from endogenous retroviral sequences driven by the long terminal repeat (LTR) sequences, which are usually heavily methylated and inactivated. Proteins such as RIG-I and MDA5 sense the presence of dsRNA and interact with the MAVS anti-viral response protein at the mitochondria. MAVS mediates activation of IRF7 and induction of an antiviral state, including the secretion of interferon, which in turn contributes to reduced cell proliferation.
Stephen Baylin's Talk